Migratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel

Author

Diao, H.J. ; Yu, C.S. ; Chan, B.P.

Author_Institution

Dept. of Mech. Eng., Univ. of Hong Kong, Hong Kong, China

fYear

2010

fDate

5-9 Dec. 2010

Firstpage

231

Lastpage

234

Abstract

The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2-3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2-6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines -10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.

Keywords

cell motility; gels; molecular biophysics; proteins; 3D collagen gel; MSC migratory response; cell migration assay; chemokine induction; collagen gel encapsulated MSC; collagen type I microspheres; differentiation potential; human orbital fat derived MSC; mesenchymal stem cell; passage 2-3 OFSC; passage 2-6 OFSC; selection barrier; Fractals; Humans; Mice; Optical microscopy; Stem cells; Three dimensional displays; Tissue engineering; 3D-migrated; OFSC; migration; selection barrier;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Molecular Medicine and Engineering (NANOMED), 2010 IEEE 4th International Conference on

Conference_Location

Hong Kong/Macau

ISSN

2159-6964

Print_ISBN

978-1-61284-152-6

Type

conf

DOI

10.1109/NANOMED.2010.5749841

Filename

5749841