A System for the Determination of Image Density and Morphometry Developed for the Analysis of Malignant Nuclei

Author

Isotani, Sadao ; Fo, Nilson Brasil ; Capeli, Olimpio M. ; Sakai, Yuriko Ito ; de Albuquerque, A.R.P.L.

Author_Institution

Univ. of Sao Paulo, Sao Paulo

fYear

2007

fDate

20-22 June 2007

Firstpage

263

Lastpage

268

Abstract

This paper describes SPCIM, a system developed for the analysis of image density and morphometry of malignant nuclei. The SPCIM has been applied in analysis of images from needle aspirations specimens, cervical intraepithelial neoplasia samples, smear of normal reproductive women and uterine-cervix smears slides of HIV-positive patients. Also, the SPCIM was applied with success in the Brazilian Papanicolaou quality control system. The main modules of the program SPCIM are "Image Acquisition", "Image Saving", "Nuclei Demarcation" and "Marked Nuclei Analysis". In this paper we emphasizes the algorithms of the module "Nuclei Demarcation", for dark regions, segmentation, area threshold, counting of regions, borderline of regions and validation of regions, that were adapted to optimize the functionality of SPCIM.

Keywords

biomedical optical imaging; diseases; image segmentation; medical image processing; optical microscopy; Brazilian Papanicolaou quality control system; HIV-positive patients; cervical intraepithelial neoplasia samples; image acquisition; image density determination; image saving; image segmentation; malignant nuclei analysis; marked nuclei analysis; morphometry; needle aspirations specimens; nuclei demarcation; uterine-cervix smears; Cancer; Image analysis; Image segmentation; Indium tin oxide; Laboratories; Needles; Neoplasms; Optical microscopy; Physics; Quality control;

fLanguage

English

Publisher

ieee

Conference_Titel

Computer-Based Medical Systems, 2007. CBMS '07. Twentieth IEEE International Symposium on

Conference_Location

Maribor

ISSN

1063-7125

Print_ISBN

0-7695-2905-4

Type

conf

DOI

10.1109/CBMS.2007.14

Filename

4262660