Nanoporous aluminum anodic oxide-based optical biosensor for real-time detection of Troponin T

A highly sensitive and real-time monitoring biosensor detecting Troponin T was developed using a nanoporous aluminum anodic oxide (AAO) layer as a sensing membrane. Troponin T is known as an unusual myocardial injury marker, and as a protein that forms the complexes with Troponin I, and Troponin C, which control muscle contraction. The device using interferometry and localized surface plasmon resonance (LSPR) phenomenon has high sensitivity and selectivity compared with a single sensing principal device. To fabricate a sensing membrane, two-step anodizing method was conducted. Au deposition was conducted to induce the LSPR phenomenon and to immobilize Troponin T antibody by SAM method. A real time sensing system was consisted of optical spectrometer and an optical fiber reflectance probe. We confirmed that the developed biosensor system had high sensitivity to detect Troponin T.