Simultaneous, accurate lifetime determination of two luminophores using time-domain techniques

The multi-luminophore lifetime determination (MLD) technique uses a simple window sum correction to calculate the true lifetime of two temporally-distinct luminophores using time-domain techniques. This method offers advantages over traditional fitting methods because it can quickly calculate lifetime for optimal use in applications which require the ability to monitor dynamic changes, such as multi-analyte sensing. Measurement error can be reduced if sampling parameters such as window width are chosen appropriately.