Title :
MEMS Coulter counter for dynamic impedance measurement of cells
Author :
Wu, Y. ; Almasri, M. ; Benson, J.D.
Author_Institution :
Dept. of Electr. & Comput. Eng., Univ. of Missouri, Columbia, MO, USA
Abstract :
In this paper, We report the design, modeling, fabrication and characterization MEMS Coulter counter which can monitor the dynamic cell impedance changes in situ as a function of time after mixing isolated cell populations with different extracellular media. The device consists of a multilayer of SU-8 microchannel which is divided into a passive mixing region, a focusing region using negative dielectrophoretic forces, and a measuring region by multiple electroplated vertical electrode pairs. Both fluidic and electrical functionality of the device were tested with using yeast cells in cryoprotectant agent (diluted dimethyl sulfoxide), and microbeads with different dimensions, and dyed fluids. The results demonstrate rapid changes of cell volume within the first 0.6 sec after mixing followed by a stable and a fixed cell volume.
Keywords :
biomedical electrodes; dyes; electrophoresis; electroplating; microfluidics; mixing; MEMS coulter counter; SU-8 microchannel multilayer; cell population isolation; cells dynamic impedance measurement; cryoprotectant agent; dyed fluid; dynamic cell impedance monitoring; extracellular media; microbead; multiple electroplated vertical electrode pair; negative dielectrophoretic force; passive mixing region; time 0.6 s; yeast cell; Electrodes; Focusing; Impedance; Impedance measurement; Microchannel; Micromechanical devices; Radiation detectors;
Conference_Titel :
Sensors, 2011 IEEE
Conference_Location :
Limerick
Print_ISBN :
978-1-4244-9290-9
DOI :
10.1109/ICSENS.2011.6127238
