PMMA/64° YX-LiNbO3 guided SH-SAW based immunosensing system

Author

Feng, Chen-Tung ; Cheng, Chi-Jung ; Atashbar, Massood Z.

Author_Institution

Electr. & Comput. Eng., Western Michigan Univ., Kalamazoo, MI, USA

fYear

2011

fDate

28-31 Oct. 2011

Firstpage

308

Lastpage

311

Abstract

In this study, a poly methylmethacrylate (PMMA)/64° YX-LiNbO₃ guided shear horizontal mode surface acoustic wave (SH-SAW) sensor was designed and fabricated for the detection of biological antigen-antibody interactions. Various thicknesses of PMMA guiding layer were spin coated on SH-SAW sensor. A test setup utilizing a small-volume flow cell with inlet and outlet ports for the microfluidic cell and employing polydimethylsiloxane (PDMS) based microfluidic channels, was also designed and fabricated using an acrylic material with a reservoir volume of 3 μl. The interactions between protein G and immunoglobulin G (IgG) solutions were measured and analyzed using the PMMA coated SH-SAW devices. The frequency shift of 64° YX-LiNbO₃ device with 0.4 μm PMMA guiding layer was measured from 3 kHz to 68 kHz as the concentration of IgG was increased from 1 μg/ml to 10 μg/ml. The calculated sensitivity of the guided SH-SAW was found to be 7.3 KHz/(μg/ml).

Keywords

biosensors; lithium compounds; microfluidics; surface acoustic wave sensors; PMMA guiding layer; SH-SAW; acrylic material; biological antigen-antibody interactions; frequency 3 kHz to 68 kHz; immunosensing system; microfluidic cell; microfluidic channels; polydimethylsiloxane; shear horizontal mode surface acoustic wave sensor; Acoustic waves; Immune system; Microfluidics; Sensitivity; Sensors; Surface acoustic wave devices;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2011 IEEE

Conference_Location

Limerick

ISSN

1930-0395

Print_ISBN

978-1-4244-9290-9

Type

conf

DOI

10.1109/ICSENS.2011.6127240

Filename

6127240