Optimization of fish gelatin-alginate-genipin as encapsulating matrices for probiotic application using FCCD-RSM

Author

Khalilah, A. K. ; Shuhaimi, M. ; Rosfarizan, M ; Arbakariya, A ; Yazid, A. M.

Author_Institution

Faculty of Applied Sciences, Universiti Teknologi MARA Malaysia, 40450 Shah Alam, Selangor, Malaysia

fYear

2012

fDate

24-27 June 2012

Firstpage

1283

Lastpage

1288

Abstract

The optimal composition of fish gelatin-alginate-genipin to encapsulate Bifidobacterium pseudocatenulatum G4 was studied. The Face Central Composition Design-Response Surface Methodology (FCCD-RSM) was employed to determine the optimal ration of the matrices in order to obtain high in encapsulation yield (%) and beads strength. Results indicated that optimized matrices could increase the entrapped viable cells and beads strength. The optimal combination of encapsulating matrices was found to be 12.57 % for fish gelatin combined with 5 % alginate and 19.1 mM genipin. Verification experiment confirmed the prediction with low value error and gave indication a good performance prognosis of the optimal formulation.

Keywords

Encapsulation; Equations; Marine animals; Mathematical model; Microorganisms; Predictive models; Response surface methodology; FCCD-RSM; Optimization; alginate; encapsulation; fish gelatin; genipin; probiotic;

fLanguage

English

Publisher

ieee

Conference_Titel

Humanities, Science and Engineering Research (SHUSER), 2012 IEEE Symposium on

Conference_Location

Kuala Lumpur

Print_ISBN

978-1-4673-1311-7

Type

conf

DOI

10.1109/SHUSER.2012.6268809

Filename

6268809