Title :
Preliminary measurements of elasticity properties of lung tumor living cells for cancer detection
Author :
Demichelis, A. ; Divieto, C. ; Mortati, L. ; Sassi, M.P. ; Sassi, G.
Author_Institution :
Ist. Naz. di Ricerca Metrol. INRIM, Turin, Italy
Abstract :
Recent studies recognized the cell stiffness changes as a marker for cancer detection. Reliable and reproducible measurements of elastic modulus, mapping different cell regions are the basis for developing a new methodology for cancer detection. To this aim, a metrological characterized AFM microscope has been developed, micro- and nano-indentation of soft materials, used as elastic modulus reference, were done and a robust statistical data analysis has been performed. Contact images and force mapping of A549 living cells allowed 4% measurement relative reproducibility. A modal cell elastic modulus 0.5 kPa, was obtained. A highly spatially resolved stiffness distribution on the overall cell regions has been obtained through data analysis, realizing a powerful tool for cell mechanics analysis.
Keywords :
atomic force microscopy; biomechanics; cancer; cellular biophysics; data analysis; elastic moduli; elasticity; lung; nanoindentation; patient diagnosis; statistical analysis; tumours; A549 living cell contact images; A549 living cell force mapping; AFM microscope; cancer detection; cell mechanics analysis; cell region; cell stiffness distribution; elastic modulus reference; lung tumor living cell elasticity property measurement; modal cell elastic modulus; soft material microindentation; soft material nanoindentation; statistical data analysis; Cancer; Elasticity; Force; Lungs; Nanoscale devices; Shape; Tumors; AFM; Sylgard; cancer diagnosis; elastic modulus; metrology; tumour cell;
Conference_Titel :
Medical Measurements and Applications (MeMeA), 2015 IEEE International Symposium on
Conference_Location :
Turin
DOI :
10.1109/MeMeA.2015.7145206
