Title :
Multiphoton in vivo flow cytometry
Author :
Zhong, Cheng Frank ; Ye, Jing Yong ; Norris, Theodore B. ; Thomas, Thommey ; Cao, Zhengyi ; Myc, Andrzej ; Baker, James R.
Author_Institution :
Center for Ultrafast Opt. Sci., Michigan Univ., Ann Arbor, MI, USA
Abstract :
Flow cytometry using two-photon excitation is demonstrated for detection of particles and cells circulating in the blood stream of a live mouse. With appropriate dye labeling, two-photon excitation enables quantitative cytometry in vivo.
Keywords :
blood flow measurement; cellular biophysics; dyes; two-photon processes; blood stream; dye labeling; multiphoton in vivo flow cytometry; two-photon excitation; Biomedical imaging; Biomedical optical imaging; Blood vessels; Cells (biology); Fluorescence; Image motion analysis; In vivo; Laser excitation; Mice; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202397
