Title :
Miniature fluorescent metabolometer
Author :
Ao, Tao ; Zhou, Lanlan ; Chance, Britton
Author_Institution :
Dept. of Biophys. & Biochem., Pennsylvania Univ., Philadelphia, PA, USA
Abstract :
The authors describe a non-intrusive method for monitoring the fluorescent reduced nicotinamide adenine dinucleotide (NADH) and the reduced flavin adenine dinucleotide (FADH2) fluorescence intensities. The ratio of the fluorescence intensities gives a measure of the oxidation-reduction (redox) state of the tissue. A yeast model has been established to calibrate the instrument.
Keywords :
bio-optics; biochemistry; biological techniques; biological tissues; bolometers; calibration; enzymes; fluorescence; molecular biophysics; oxidation; reduction (chemical); calibration; flavin adenine dinucleotide; fluorescence intensities; fluorescent reduced nicotinamide adenine dinucleotide; miniature fluorescent metabolometer; nonintrusive method; oxidation-reduction state; tissue; yeast; Biomedical optical imaging; Fluorescence; Fungi; Humans; Instruments; Laboratories; Light emitting diodes; Monitoring; Probes; Signal processing;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202426
