Title :
Study of Two-Photon Microscopic Imaging of Human Colorectal Mucosa Stimulated by Femtosecond Laser
Author_Institution :
Key Lab. of Optoelectron. Sci. & Technol. for Med. of Minist. of Educ., Fujian Normal Univ., Fuzhou, China
Abstract :
Two-photon laser scanning microscopy (TPLSM) has high resolution and high contrast in imaging modality, with many advantages such as non-invasive, no-toxicity, and nophotobleaching and so on. We demonstrated the influence of excitation wavelength and excitation power on the SHG and TPEF signals of human rectal mucosa, and the spectral information of certain intrinsic components in mucosa was obtained and discussed. In summary, our results demonstrate that TPLSM is a feasible tool for microscopic analysis of intestinal mucosa morphometry in vivo.
Keywords :
biological tissues; biomedical optical imaging; fluorescence spectroscopy; infrared spectroscopy; optical harmonic generation; optical microscopy; two-photon spectroscopy; TPLSM; excitation power effects; excitation wavelength effects; femtosecond laser stimulation; human colorectal mucosa; human rectal mucosa SHG signals; human rectal mucosa TPEF signals; imaging modality; in vivo intestinal mucosa morphometry; microscopic analysis; two photon laser scanning microscopy; two photon microscopic imaging; Humans; In vivo; Laser excitation; Microscopy; Skin;
Conference_Titel :
Photonics and Optoelectronics (SOPO), 2012 Symposium on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4577-0909-8
DOI :
10.1109/SOPO.2012.6270961
