Micropatterning of bioactive ligands for high-throughput study neuronal network functions

Author

Li Wei ; Xu Zhen ; Peng Shi

Author_Institution

Dept. of Mech. & Biomed. Eng., City Univ. of Hong Kong, Hong Kong, China

fYear

2013

fDate

5-8 Aug. 2013

Firstpage

170

Lastpage

173

Abstract

Synapses are essential components for signal processing and transmission in the nervous system, whose function is affected in many brain diseases and disorders. We demonstrated the successful control of synapse formation by micropatterning of synaptogenic protein, neuroligin (NL). Interestingly, the presence of a hydrophobic GPI moiety, promoted NL´s biological activity. Also, the micropatterns of synaptogenic factors were included in a neurite growth/guidance platform, which could directly be used as a high-throughput platform for study different aspects in the development and dynamics of neuronal network.

Keywords

biochemistry; bioelectric potentials; brain; cellular biophysics; hydrophobicity; molecular biophysics; neural nets; neurophysiology; proteins; bioactive ligand micropatterning; brain diseases; brain disorders; high-throughput platform; hydrophobic GPI moiety; nervous system; neurite growth platform; neurite guidance platform; neuroligin biological activity; neuroligin micropatterning; neuronal network functions; signal processing; signal transmission; synapse formation control; synaptogenic protein micropatterning; Biological neural networks; Nerve fibers; Phase locked loops; Protein engineering; Proteins; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2013 13th IEEE Conference on

Conference_Location

Beijing

ISSN

1944-9399

Print_ISBN

978-1-4799-0675-8

Type

conf

DOI

10.1109/NANO.2013.6720879

Filename

6720879