Fluctuation tolerant read scheme for ultrafast DNA sequencing with nanopore device

A charge-integration read scheme has been developed for a solid-nanopore DNA-sequencer that determines a genome by direct and electrical measurements of tunneling current in a single-stranded DNA. Although the tunneling current varies depending on the translational and rotational movement of the DNA, the developed scheme cumulates charge at the parasitic capacitance of the nanopore device so that the base-types can be distinguished even under such noisy condition. A circuit simulation demonstrates that the scheme successfully distinguishes between each DNA base at the speed of 2.0 ms/base. The speed is roughly six orders faster than that of a conventional DNA sequencer. With another scheme which reads complementary base-pair simultaneously with two nanopores, the speed is further improved, and the human genome can be sequenced in only one day if 100 nanopore systems operate in parallel with the scheme.