Title :
Mixed Experimental Methodologies to Optimize Biosurfactant Production Conditions of Pseudomonas Aeruginosa
Author :
Tian, Jing ; Wang, Jing ; Zhou, Yong-Jun ; Dong, Han-Ping ; Yu, Li
Author_Institution :
Fac. of Chem. Eng., China Univ. of Pet., Beijing, Beijing, China
Abstract :
In order to reduce production cost of Pseudomonas aeruginosa T1, which has the ability of producing the biosurfactant when using crude oil as the sole carbon source, Three experimental methodologies (Plackett-Burman design (PBD), steepest ascent design (SAD), Central composite rotatable design (CCRD)) were applied to optimize the medium ingredients of the strain. In the verification experiment, the surface tension (ST) of strain T1 fermentation decreased from 29.93mN/m to 27.08mN/m after medium optimization.
Keywords :
biotechnology; microorganisms; surface tension; CCRD; PBD; Plackett-Burman design; Pseudomonas aeruginosa T1; SAD; biosurfactant production conditions; central composite rotatable design; crude oil; experimental methodologies; sole carbon source; steepest ascent design; strain T1 fermentation; surface tension; Biological system modeling; Equations; Mathematical model; Petroleum; Production; Response surface methodology; Strain; Central composite rotatable design; Plackett-Burman design; Pseudomonas aeruginosa; Steepest ascent design;
Conference_Titel :
Cryptography and Network Security, Data Mining and Knowledge Discovery, E-Commerce & Its Applications and Embedded Systems (CDEE), 2010 First ACIS International Symposium on
Conference_Location :
Qinhuangdao
Print_ISBN :
978-1-4244-9595-5
DOI :
10.1109/CDEE.2010.74
