Mixed Experimental Methodologies to Optimize Biosurfactant Production Conditions of Pseudomonas Aeruginosa

Author

Tian, Jing ; Wang, Jing ; Zhou, Yong-Jun ; Dong, Han-Ping ; Yu, Li

Author_Institution

Fac. of Chem. Eng., China Univ. of Pet., Beijing, Beijing, China

fYear

2010

fDate

23-24 Oct. 2010

Firstpage

358

Lastpage

361

Abstract

In order to reduce production cost of Pseudomonas aeruginosa T1, which has the ability of producing the biosurfactant when using crude oil as the sole carbon source, Three experimental methodologies (Plackett-Burman design (PBD), steepest ascent design (SAD), Central composite rotatable design (CCRD)) were applied to optimize the medium ingredients of the strain. In the verification experiment, the surface tension (ST) of strain T1 fermentation decreased from 29.93mN/m to 27.08mN/m after medium optimization.

Keywords

biotechnology; microorganisms; surface tension; CCRD; PBD; Plackett-Burman design; Pseudomonas aeruginosa T1; SAD; biosurfactant production conditions; central composite rotatable design; crude oil; experimental methodologies; sole carbon source; steepest ascent design; strain T1 fermentation; surface tension; Biological system modeling; Equations; Mathematical model; Petroleum; Production; Response surface methodology; Strain; Central composite rotatable design; Plackett-Burman design; Pseudomonas aeruginosa; Steepest ascent design;

fLanguage

English

Publisher

ieee

Conference_Titel

Cryptography and Network Security, Data Mining and Knowledge Discovery, E-Commerce & Its Applications and Embedded Systems (CDEE), 2010 First ACIS International Symposium on

Conference_Location

Qinhuangdao

Print_ISBN

978-1-4244-9595-5

Type

conf

DOI

10.1109/CDEE.2010.74

Filename

5759347