Extraction of isothiocyanate in Wasabi Japonica Matsum and its effects to influenza virus

With a view to develop medicinal value of Wasabi Japonica Matsum, isothiocyanate, the active ingredient, were extracted by extraction and distillation methods, the extracted isothiocyanate had then been tested on effects against influenza virus by resorting to hemagglutination test and animal experiment methods in getting the content and effects of isothiocyanate in Wasabi Japonica Matsum. The results show that the content of isothiocyanate in Wasabi Japonica Matsum were 0.53mol·mL-1 in fibrous root, 0.80mol·mL-1 in rhizome and 0.27mol·mL-1 in petiole. Moreover isothiocyanate in Wasabi Japonica Matsum had remarkable effects in killing influenza virus. When the proportion of isothiocyanate and virus was O.1SmL +0.05mL, it enabled virus to lost activity completely after being treated for 90min, let alone diseases could be caused. This study provides scientific basis for isothiocyanate extraction in Wasabi Japonica Matsum and its medicinal value.