Tuberculosis bacilli detection in Ziehl-Neelsen-stained tissue using affine moment invariants and Extreme Learning Machine

This paper describes an approach to automate the detection and classification of tuberculosis (TB) bacilli in tissue section using image processing technique and feedforward neural network trained by Extreme Learning Machine. It aims to assist pathologists in TB diagnosis and give an alternative to the conventional manual screening process, which is time-consuming and labour-intensive. Images are captured from Ziehl-Neelsen (ZN) stained tissue slides using light microscope as it is commonly used approach for diagnosis of TB. Then colour image segmentation is used to locate the regions correspond to the bacilli. After that, affine moment invariants are extracted to represent the segmented regions. These features are invariant under rotation, scale and translation, thus useful to represent the bacilli. Finally, a single layer feedforward neural network (SLFNN) trained by Extreme Learning Machine (ELM) is used to detect and classify the features into three classes: `TB´, `overlapped TB´ and `non-TB´. The results indicate that the ELM gives acceptable classification performance with shorter training period compared to the standard backpropagation training algorithms.