Title :
Formaldehyde degradation filter via recombinant E. coli enzyme
Author :
Yamaguchi, Masaki ; Tahara, Yusuke ; Kanemaru, Masashi ; Deguchi, Mitsuo ; Ozawa, Satoshi ; Arar, Jun-ichiro
Author_Institution :
Fac. of Eng., Toyama Univ., Japan
Abstract :
In order to develop a bioremediation process to degrade formaldehyde gas from house interiors, a formaldehyde degradation filter using immobilized recombinant FDH protein was fabricated. As the results, we have successfully cloned the FDH gene into E.coli and extracted highly purified recombinant FDH protein of 12.4 kU/L. Using the extracted recombinant FDH protein and immobilizing it on a filter we have experimentally verified the degradation capability of such a filter for formaldehyde gas for bioremediation.
Keywords :
biochemistry; cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; FDH gene; bioremediation; formaldehyde degradation filter; formaldehyde gas; immobilized recombinant FDH protein; recombinant E. coli enzyme; Biochemistry; Cells (biology); Cloning; DNA; Degradation; Energy consumption; Filters; Ice; Microorganisms; Protein engineering;
Conference_Titel :
Biomedical Engineering, 2003. IEEE EMBS Asian-Pacific Conference on
Print_ISBN :
0-7803-7943-8
DOI :
10.1109/APBME.2003.1302606
