Pricise Titration Microfluidic System using Sub-Picoliter Droplet Injection

Precise titration using sub-picoliter droplet injection microfluidic system is proposed. This system can to measure less than 1 picoliter of reagent and to deliver to the micro chamber without diffusion which becomes major concern when the reagent volume is smaller. A prototype device can generate a droplet of 0.8 picoliter (800 femtoliter) in the metering channel of 40 mum times 5 mum times 4 mum, and step-by-step titration by precise reagent injection per two hundred thousand (2.0times10^-5) in volume of the micro chamber (157 nanoliter: 1 mm in diameter times 200 mum in height).