Detection for Cleavage Product of Restriction Enzyme Using Molecular-Beacon-Ligation System

A new approach for detection cleavage product of restriction enzyme was reported based on molecular-beacon- ligation system. The molecular beacon, designed in such a way that one-half of its loop is complementary to cleavage product, is used to monitor ligation process of nucleic acids complex and to convert directly cleavage product information into fluorescence signal. The method is fast, simple and ultrasensitive for detection cleavage product. Detection limit of the assay is 0.05 nmol/L. moreover; the method need not label restriction enzyme and substrate. The cleavage product of Bst1107 restriction enzyme has been detected based on this assay.