Title :
Automatic segmentation and modelling of two-dimensional electrophoresis gels
Author :
Bettens, E. ; Scheunders, P. ; Sijbers, J. ; Van Dyck, D. ; Moens, L.
Author_Institution :
Dept. of Phys., Antwerp Univ., Belgium
Abstract :
An important issue in the analysis of two-dimensional electrophoresis images is the detection and quantification of protein spots. In this paper we describe a new robust technique to segment and model the different spots present in the gels. For the segmentation a watershed technique is applied. For the quantification of the spots, a new spot model is constructed, based on diffusion principles. Besides the advantage of having a physical interpretation, the model is demonstrated to be superior to the commonly used Gaussian models
Keywords :
biological techniques; biology computing; electrophoresis; gels; image recognition; image segmentation; proteins; automatic segmentation; detection; diffusion; protein spots; quantification; robust technique; spot model; two-dimensional electrophoresis gels; Diffusion processes; Electrokinetics; Image analysis; Image processing; Image segmentation; Pattern analysis; Pattern recognition; Physics; Proteins; Robustness;
Conference_Titel :
Image Processing, 1996. Proceedings., International Conference on
Conference_Location :
Lausanne
Print_ISBN :
0-7803-3259-8
DOI :
10.1109/ICIP.1996.560965
