Primary study on thermophiles during pile-fermentation of Pu-er tea

Author

Chen, Chaoyin ; Zhang, Wenming ; Chanyong Lu ; Han, Benyong ; Xiong, Xianfeng ; Ge, Feng ; Zhao, Shenglan ; Chen, D.

Author_Institution

Fac. of Life Sci. & Technol, Kunming Univ. of Sci. & Technol., Kunming, China

Volume

2

fYear

2011

fDate

9-11 Dec. 2011

Firstpage

691

Lastpage

695

Abstract

Pu-er tea pile-fermentation is a high temperature process. Thermophiles during the pile-fermentation of Pu-er tea have not been reported up to now. This paper was isolated 13 thermophiles during the pile-fermentation of Pu-er. Then each strain was inoculated into the piles of Pu-er tea and simulated thermophilic pile-fermentation, with testing tea polyphenols, theaflavin, thearubigins and theabrownines. The content of theabrowin of the group added the 13 strains greatly increased to 22.73%. Six more favorable and important strains were identified by 16S rDNA sequencing. The results indicated that three belong to Bacillus, the others to Thermoactinomyces, Streptomyces, Ureibacillus, respectively.

Keywords

beverages; fermentation; microorganisms; 16S rDNA sequencing; Bacillus; Pu-er tea; Streptomyces; Thermoactinomyces; Ureibacillus; high-temperature process; polyphenols; simulated thermophilic pile-fermentation; theabrowin content; theabrownines; theaflavin; thearubigins; thermophiles; Microorganisms; Pigments; Strain; Surface morphology; Surface treatment; Temperature measurement; Turning; Pile-Fermentation; Pu-er tea; Thermophiles;

fLanguage

English

Publisher

ieee

Conference_Titel

IT in Medicine and Education (ITME), 2011 International Symposium on

Conference_Location

Cuangzhou

Print_ISBN

978-1-61284-701-6

Type

conf

DOI

10.1109/ITiME.2011.6132094

Filename

6132094