Localization of S-lap/GFP fusion protein and its effect on cell cycle in 293T cells

The s-lap is a novel gene discovered and cloned from the porcine retinal pigment epithelial cells which show earlier signs of apoptosis induced by visible light damage. However, its function is still unclear. To clarify the relationship between s-lap and apoptosis, we investigated the expression, localization of s-lap/GFP fusion protein and its effect on cell cycle in 293T cells. The plasmid expressing s-lap/GFP fusion protein was constructed and transfected into 293T cells. Then the expression and localization of s-lap/GFP fusion protein was observed by fluorescence microscope, and the effect on cell cycle were evaluated by FACS. The result shown that The recombinant plasmid can express s-lap/GFP fusion protein in 293T cells. The observation with fluorescence microscope showed that distributed of s-lap/GFP was shared in the entire cell but the priority of which was given to the nuclear, while the single GFP distributed in the cytoplasm. The cell cycle result indicated that the cell number in G2/M phase increased dramatically after s-lap/GFP transfection.