Title :
Multi-resistance analysis of secreted peroxidase from wintersweet (Chimonanthus praecox)
Author :
Li-Hong Zhang ; Gao, Chang-Cheng ; Yang, Xiao-Hong ; Zou, Xian-Feng ; Wu, Qiong ; Chen, Xing ; Chen, Li-Na ; Yu, Shu-Yan ; Zhang, Shi-Hong
Author_Institution :
Key Lab. of Agric. Products Process., Changchun Univ., Changchun, China
Abstract :
Peroxidases play a critical role in the anti-oxidant enzyme protection system of plants accordingly, we assumed that wintersweet (Chimonanthus praecox), as a multi-resistant plant adapting to a variety of stressful conditions, should contain certain special peroxidase. In this study, a putative peroxidase cDNA containing a ORF (CpPOD) was cloned by screening the full-length cDNA library of wintersweet. In order to decipher the its function, the cloned gene was overexpressed in tobacco system via Agrobacterium-mediated transformation method. The results initially showed that overexpression of CpPOD can not only enhance the ability of water-keeping, but also provide anti-oxidant activity to stress injury. All these results suggested that the CpPOD gene presents multiple resistance actions.
Keywords :
DNA; enzymes; genetics; microorganisms; molecular biophysics; Agrobacterium-mediated transformation method; antioxidant enzyme protection system; cloned gene; multiresistance analysis; putative peroxidase cDNA; secreted peroxidase; stress injury; tobacco system; water-keeping; wintersweet (Chimonanthus Praecox); Cloning; Libraries; Pathogens; Proteins; Strain; Stress; Vectors; multi-resistance; peroxidase; wintersweet(Chimonanthus praecox);
Conference_Titel :
IT in Medicine and Education (ITME), 2011 International Symposium on
Conference_Location :
Cuangzhou
Print_ISBN :
978-1-61284-701-6
DOI :
10.1109/ITiME.2011.6132100