DocumentCode
3048424
Title
Multi-resistance analysis of secreted peroxidase from wintersweet (Chimonanthus praecox)
Author
Li-Hong Zhang ; Gao, Chang-Cheng ; Yang, Xiao-Hong ; Zou, Xian-Feng ; Wu, Qiong ; Chen, Xing ; Chen, Li-Na ; Yu, Shu-Yan ; Zhang, Shi-Hong
Author_Institution
Key Lab. of Agric. Products Process., Changchun Univ., Changchun, China
Volume
2
fYear
2011
fDate
9-11 Dec. 2011
Firstpage
715
Lastpage
719
Abstract
Peroxidases play a critical role in the anti-oxidant enzyme protection system of plants accordingly, we assumed that wintersweet (Chimonanthus praecox), as a multi-resistant plant adapting to a variety of stressful conditions, should contain certain special peroxidase. In this study, a putative peroxidase cDNA containing a ORF (CpPOD) was cloned by screening the full-length cDNA library of wintersweet. In order to decipher the its function, the cloned gene was overexpressed in tobacco system via Agrobacterium-mediated transformation method. The results initially showed that overexpression of CpPOD can not only enhance the ability of water-keeping, but also provide anti-oxidant activity to stress injury. All these results suggested that the CpPOD gene presents multiple resistance actions.
Keywords
DNA; enzymes; genetics; microorganisms; molecular biophysics; Agrobacterium-mediated transformation method; antioxidant enzyme protection system; cloned gene; multiresistance analysis; putative peroxidase cDNA; secreted peroxidase; stress injury; tobacco system; water-keeping; wintersweet (Chimonanthus Praecox); Cloning; Libraries; Pathogens; Proteins; Strain; Stress; Vectors; multi-resistance; peroxidase; wintersweet(Chimonanthus praecox);
fLanguage
English
Publisher
ieee
Conference_Titel
IT in Medicine and Education (ITME), 2011 International Symposium on
Conference_Location
Cuangzhou
Print_ISBN
978-1-61284-701-6
Type
conf
DOI
10.1109/ITiME.2011.6132100
Filename
6132100
Link To Document