The primary culture of vascular endothelial cell in rat

The purpose of the experiment: to explore the primary cultural method of vascular endothelial cell in rat that we have got through obtained and cultivated, the experiment were provided to the corresp technique of the further research. Experimental methods: the aorta of Wistar rat was obtained in operating microscope, then removal the adventitia and adipose tissue. The blood vessel were sheared like the organization by 1mm×1mm. Using stationary culture in DMEM/F12 culture medium contained fetal bovine serum (FBS). The results of experiment: endothelial cell swimmed from the borderline of organization to grow by adherence after three days. Then the cell began to synthesis after 10 to 12 days and were confluenced to monolayer about 70%. The passage cell were grew prosperity from the digest of diastase vera and were converged like the cobblestone characteristic of endothelial cell. The method of experiment was convenient and economy, we can obtain vascular endothelial cell in high fineness without collagenase and the supplement of endothelial cell in cell growth.