Title :
Protein Diffusion through Polymer Meshes As Observed by Pulsed-Field Gradient NMR
Author :
Guo, Xuhong ; Li, Li ; Pacheco, Carlos R. ; Fu, Lin ; Prud´homme, R.K.
Author_Institution :
State-Key Lab. of Chem. Eng., East China Univ. of Sci. & Technol., Shanghai
Abstract :
Pulsed-field gradient NMR was employed to observe the diffusion of alpha-lactalbumin in poly(ethylene oxide) (PEO) solutions of deuterated water. Polymer mesh size and the interactions between protein and polymer networks strongly affect protein diffusion through polymer meshes. The diffusion coefficients determined by pulsed-field gradient NMR show a good agreement at low polymer concentrations with those obtained by neutron spin-echo spectroscopy (NSE). Deviation between experimental data from theoretical prediction comes from the interactions between protein and polymer meshes.
Keywords :
biochemistry; biological NMR; molecular biophysics; neutron spin echo; polymers; proteins; alpha-lactalbumin; deuterated water; neutron spin-echo spectroscopy; polyethylene oxide solutions; polymer meshes; protein diffusion; pulsed-field gradient NMR; Area measurement; Chemical engineering; Laboratories; Neutron spin echo; Nuclear magnetic resonance; Pollution measurement; Polymers; Protein engineering; Pulse measurements; Spectroscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2007. ICBBE 2007. The 1st International Conference on
Conference_Location :
Wuhan
Print_ISBN :
1-4244-1120-3
DOI :
10.1109/ICBBE.2007.184
