Development of species-specific SCAR (sequence-characterized amplified region) markers for identification of famous-region drug Ophiopogon japonicus (L.f.) Ker-Gawl

Author

Zhu, Zhen-Hong ; Ge, Li-Jun ; Wang, Yu

Author_Institution

Coll. of Bioeng., Zhejiang Chinese Med. Univ., Hangzhou, China

Volume

2

fYear

2011

fDate

9-11 Dec. 2011

Firstpage

633

Lastpage

637

Abstract

Correct genotype identification of medicinal plant material remains important for Chinese traditional medicine. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in discrimination of famous-region herbal medicines. The present study was carried out to develop DNA based markers for identification of Chinese famous-region drug Ophiopogon japonicus (L.f.) Ker-Gawl. Two specific RAPD fragments R01 (268bp) and R02 (1364bp) for famous-region drug Ophiopogon japonicus (L.f.) Ker-Gawl were identified by Random Amplified Polymorphic DNA (RAPD). Sequence Characterized Amplified Region (SCAR) markers (248bp & 631bp) were developed from the RAPD amplicon. The SCAR markers were found useful for identification of genuine regional Ophiopogon japonicus ( L.f. ) Ker-Gawl in four different populations.

Keywords

DNA; biomedical materials; drugs; molecular biophysics; organic compounds; Chinese famous-region drug Ophiopogon japonicus Ker-Gawl; Chinese traditional medicine; DNA based markers; RAPD fragments; authentication; chemical approaches; correct genotype identification; famous-region herbal medicines; genuine regional Ophiopogon japonicus Ker-Gawl; medicinal plant material; morphological approaches; random amplified polymorphic DNA; sequence characterized amplified region markers; species-specific SCAR markers; Ophiopogon japonicus (L.f.) Ker-Gawl; RAPD; SCAR marker; famous-region drug; identification;

fLanguage

English

Publisher

ieee

Conference_Titel

IT in Medicine and Education (ITME), 2011 International Symposium on

Conference_Location

Cuangzhou

Print_ISBN

978-1-61284-701-6

Type

conf

DOI

10.1109/ITiME.2011.6132190

Filename

6132190