A vertically aligned carbon nanofiber (VACNF) based amperometric glucose sensor

The use of vertically aligned carbon nanofibers has been found to be an effective strategy for building a biosensor platform. The VACNF structure is conducive to immobilization because of the presence of many defect sites that have exposed carboxyl groups which allow successful immobilization of the biological elements. The abundance of defect sites is not only good for immobilization but also for strong electrical current response. In this study, a mediator free amperometric bienzyme glucose biosensor is constructed by co-immobilization of horseradish peroxidase and glucose oxidase on the vertically aligned carbon nanofiber electrode. Reduction peaks have been observed due to presence of horseradish peroxidase at the VACNF electrode. High sensitivity of this biosensor to glucose has also been observed. Linear range has been found up to glucose concentration of 40 ¿M.