Title :
Simultaneous 3D imaging of morphology and nanoparticle distribution in single cells with the Cell-CT™ technology
Author :
Neumann, Thomas ; Miao, Qin ; Yu, Julia ; Fauver, Mark ; Meyer, Michael ; Rahn, J.Richard ; Lancaster, Christy A. ; Seibel, Eric J. ; Nelson, Alan C.
Author_Institution :
VisionGate Inc., Gig Harbor, WA 98335 USA
Abstract :
The Cell-CT™ is an optical projection tomography microscope (OPTM) developed for high resolution 3D imaging of single cells based on absorption stains and brightfield microscopy. In this study we demonstrate the use of the Cell-CT™ in multi-color mode for simultaneous imaging of cellular 3D morphology and the 3D distribution of nanoparticle clusters in the cytoplasm. The ability to image cellular processes in relation to cellular compartments with a non-fluorescence 3D technology opens new perspectives for molecular research.
Keywords :
High-resolution imaging; Image resolution; Morphology; Nanoparticles; Optical imaging; Optical microscopy; Optical refraction; Optical sensors; Optical variables control; Tomography; Algorithms; Animals; Cell Line, Tumor; Cell Size; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Lung Neoplasms; Nanoparticles; Particle Size; Rats; Reproducibility of Results; Sensitivity and Specificity; Subcellular Fractions; Tomography, Optical;
Conference_Titel :
Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE
Conference_Location :
Vancouver, BC
Print_ISBN :
978-1-4244-1814-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2008.4649169
