DocumentCode
3068179
Title
Dependence of the photobleaching of fluorescent proteins on the repetition rate of femtosecond light pulses
Author
Toda, Keisuke ; Takahashi, Hiroki ; Suda, Akira
Author_Institution
Dept. of Phys., Tokyo Univ. of Sci., Noda, Japan
fYear
2013
fDate
June 30 2013-July 4 2013
Firstpage
1
Lastpage
2
Abstract
We investigate the photobleaching of fluorescent proteins as functions of pulse interval, chirp, and pulse energy of femtosecond excitation lights and find out that the photobleaching occurs via one-photon excited-state absorption of the triplet state.
Keywords
fluorescence; high-speed optical techniques; molecular biophysics; optical saturable absorption; proteins; femtosecond excitation light; femtosecond light pulse; fluorescent proteins; one-photon excited-state absorption; photobleaching; pulse chirp; pulse energy; pulse interval; repetition rate; triplet state; Absorption; Chirp; Delays; Fluorescence; Microscopy; Photobleaching; Proteins;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics Pacific Rim (CLEO-PR), 2013 Conference on
Conference_Location
Kyoto
Type
conf
DOI
10.1109/CLEOPR.2013.6600622
Filename
6600622
Link To Document