Title :
Fiber-optic endomicroscopy system for high-resolution nonlinear imaging of biological tissue
Author :
Wu, Yicong ; Xi, Jiefeng ; Cobb, Michael J. ; Xingde Li
Author_Institution :
Department of Bioengineering, University of Washington, Seattle, 98195, USA
Abstract :
A flexible fiber-optic endomicroscopy system was developed for nonlinear optical imaging of biological samples. Double-clad fiber and photonic bandgap fiber were employed in the endomicroscope for femtosecond pulse delivery, dispersion compensation, nonlinear optical signals collection and fast beam scanning. Three-dimensional imaging of biological tissues based on second harmonic generation and two-photon excited fluorescence was performed with the endomicroscope. The depth-resolved imaging results strongly suggest the potential of this flexible fiber-optic microscope system for real-time assessment of both epithelial and stromal structures in luminal organs.
Keywords :
Biological tissues; Biomedical optical imaging; Fiber nonlinear optics; Fluorescence; High-resolution imaging; Optical harmonic generation; Optical imaging; Optical pulses; Photonic bandgap fibers; Ultrafast optics; Animals; Biomedical Engineering; Endoscopes; Imaging, Three-Dimensional; Microscopy; Nonlinear Dynamics; Optical Fibers; Rats; Signal Processing, Computer-Assisted; Swine;
Conference_Titel :
Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE
Conference_Location :
Vancouver, BC
Print_ISBN :
978-1-4244-1814-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2008.4649541
