Multiphoton endoscope using MEMS scanner

Multiphoton Microscopy (MPM) is a non-destructive, high-resolution, optical imaging technique with demonstrated ability in thick tissues. MPM systems need femtosecond lasers for excitation of nonlinear optical signals and thus are usually developed using free-space optics. However, for in vivo imaging and clinical applications, a fiberoptic-based MPM endoscope is desirable because light can be delivered with a flexible fiber and images can be acquired with a miniature probe. The challenges for MPM endoscopy include the delivery of femtosecond optical pulses through optical fiber and the miniaturization of the scanning probe head. We will show the development of a MPM endoscope using photonic crystal fiber and 2-axis MEMS scanner.