A nonenzymatic biosensor based on copper nanoparticles modified electrode for detection of acetylcholine

The electrocatalytic oxidation of acetylcholine (Ach) on copper microparticles modified carbon paste electrode (m-CPE) and copper nanoparticles-modified carbon paste electrode (n-CPE) was investigated. In the voltammogram recorded using m-CPE, a single anodic peak related to the oxidation of ACh was observed. Using n-CPE, however, two overlapped anodic peaks were observed. The amino acids oxidized on n-CPE at higher rates at lower potentials compared to m-CPE. A nonenzymatic amperometric biosensor was constructed and a simple analysis procedure was developed for the determination of ACh.