Tracking the Intracellular Dynamics of Transferrin-labeled QDs in Living Panc-1 Cells

Author

Xu, Gaixia ; Qu, Junle ; Jaeger, Stefan ; Song, Qingfeng ; Chen, Su-Shing

Author_Institution

Key Lab. of Optoelectron. Devices & Syst. of Minist. of Educ., Shenzhen Univ., Shenzhen, China

fYear

2009

fDate

22-24 June 2009

Firstpage

330

Lastpage

334

Abstract

We have used Quantum dots (QD) bioconjugated with Transferrin (Tf) as targeted luminescent probes to label pancreatic cancer cells, and have employed a confocal microscope to localize the distribution and track the dynamics of QD-Tf nanocomplexes. In this paper, we present our recent results on QD-Tf tracking and vesicle imaging, including quasi-real-time visualization of QD-Tf transport inside cells using a tracking algorithm. This work demonstrates that we have a potentially useful method for discovering the molecular delivery mechanism inside living cells by utilizing the QDs bioconjugates in combination with our tracking algorithm.

Keywords

biomedical optical imaging; cancer; cellular biophysics; medical image processing; molecular biophysics; nanobiotechnology; optical microscopy; optical tracking; quantum dots; confocal microscope; intracellular dynamics tracking; living Panc-1 cells; molecular delivery mechanism; nanomedicine; pancreatic cancer cells; quantum dot bioconjugates; quasireal-time visualization; targeted luminescent probes; tracking algorithm; transferrin-labeled quantum dots; vesicle imaging; Fluorescence; Immune system; Iron; Microorganisms; Nanobioscience; Nanoparticles; Neoplasms; Probes; Quantum dots; Target tracking; imaging; nanomedicine; quantum dots; tracking;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and BioEngineering, 2009. BIBE '09. Ninth IEEE International Conference on

Conference_Location

Taichung

Print_ISBN

978-0-7695-3656-9

Type

conf

DOI

10.1109/BIBE.2009.29

Filename

5211253