Author_Institution :
Coll. of Environ. & Chem. Eng., Yanshan Univ., Qinhuangdao, China
Abstract :
1H NMR-based metabonomic method was used to investigate the biochemical variability in serum from rats treated by nephrotoxins. The toxic effect of the model toxins, sodium chromate (Na2CrO4, 20 mg/kg body mass), 2-bromoethanamine hydrobromide (BEA, 250 mg/kg body mass) and mercury II chloride (HgCl2, 1 mg/kg body mass), was evaluated by 1H NMR spectroscopy accompanied by principal components analysis (PCA) method. The result showed that common changes in these 1H NMR spectra profile of serum included elevation in levels of LDL, VLDL, lactate, creatinine, fatty acyl as well as decrease of glucose, which could be serum NMR markers of renal toxicity. The additional changes in the NMR spectroscopy brought by HgCl2 included increase of acetate, acetone acetoacetate and decrease of taurine signals, which were mainly related to its slight hepatotoxicity. The above results were supported by clinical chemistry parameters, and the main serum biomarkers of renal toxicity recognized in the NMR spectral profiles were conformed to the results of PCA. The pilot studies demonstrate that the combination of 1H NMR and pattern recognition analysis is an effective method in biological studies and might provide further information in toxicological study.
Keywords :
biochemistry; biomedical NMR; liver; medical signal processing; mercury compounds; molecular biophysics; pattern recognition; principal component analysis; sodium compounds; toxicology; 2-bromoethanamine hydrobromide; HgCl2; LDL; NMR; Na2CrO4; VLDL; acetate; acetone acetoacetate; biochemical variability; clinical chemistry; creatinine; fatty acyl; glucose; hepatotoxicity; lactate; metabonomic method; nephrotoxins; pattern recognition; principal components analysis; renal toxicity; serum; toxins; Amino acids; Biomarkers; Chemistry; Information analysis; Mass spectroscopy; Nuclear magnetic resonance; Pattern recognition; Principal component analysis; Rats; Sugar;