Study on Methods of Protein Immobilization for AFM

Author

Wang, Jianhua ; Wang, Congzhou

Author_Institution

Coll. of Bioeng., Chongqing Univ., Chongqing, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

The potentialities of the atomic force microscopy (AFM) make it a tool of the undeniable value for the study of biomolecule samples. One of the main steps for the application of atomic force microscopy to the study of proteins has been the self-assembled monolayers (SAMs). The methods of physical adsorption and chemical modification are utilized to immobilize proteins to substrates efficiently. SAMs play an important role in protein immobilization. Membrane proteins need especial immobilization procedures in order to maintain their biological activities in vitro. This review proposes some fundamental methods of protein immobilization for AFM study.

Keywords

adsorption; atomic force microscopy; biochemistry; biological techniques; biomembranes; monolayers; proteins; proteomics; self-assembly; AFM; atomic force microscopy; biological activities; biomolecule samples; chemical modification; membrane proteins; physical adsorption; protein immobilization; self-assembled monolayers; Atomic force microscopy; Atomic layer deposition; Biomembranes; Bovine; Chemicals; Coatings; Electrostatics; Proteins; Scanning electron microscopy; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5514974

Filename

5514974