Study on Reversibility of Genetic Toxicity of TDI in Mice

Author

Ji, Yu-bin ; Yu, Miao ; Yu, Lei ; Yang, Wen ; Yang, Ying-jie

Author_Institution

Center of Res. on Life Sci. & Environ. Sci., Harbin Univ. of Commerce, Harbin, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

This article studied whether genetic toxicity of toluene diisocyanate was reversible. Kunming mice were exposed to TDI by respiratory tract for 14 days in 1/8LC₅₀, 1/4LC₅₀ and 1/2 LC₅₀ dosage and then were feed normally for 14 days. The chromosomal aberrations and the content of RNA & DNA were detected by optical microscope and laser scanning confocal microscope. In 1/4LC₅₀ and 1/2 LC₅₀ group, chromosome aberration rate and the RNA / DNA ratio of TDI were higher than control group significantly (P<;0.01). In addition there was no significant difference between 1/4LC₅₀ and 1/2 LC₅₀ (p> 0.05). The results showed that the damage of TDI on chromosomes and DNA was repairable, but can not be repaired completely.

Keywords

DNA; cellular biophysics; genetics; optical microscopy; toxicology; DNA; Kunming mice; RNA; chromosomal aberration; genetic toxicity; laser scanning confocal microscopy; optical microscopy; respiratory tract; reversibility; toluene diisocyanate; Biological cells; Biomedical optical imaging; Cells (biology); DNA; Fluorescence; Genetics; Mice; Optical microscopy; RNA; Sealing materials;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5515186

Filename

5515186