Title :
Purification and Some Properties of Alkaline Phosphatase from the Liver of Bos taurus
Author :
Tang Liang-ting ; Fu Wei-li ; Wang Song ; Zhu Hong ; Tang Yun-Ming
Author_Institution :
Key Lab. of Eco-environments in Three Gorges Reservoir Region Minist. of Educ., Southwest Univ., Chongqing, China
Abstract :
Alkaline phosphatase was isolated and purified from the cattle (Bos taurus ) liver by means of homogenizeation, n-butanol disposal, thermal denaturation, ultrafiltration, DEAE- Sepharose column chromatography and Sephacryl S-200 gel filtration chromatography. Its specific activity was 19863.50, and the purification fold was 228.74. Its optimum pH and temperature was 10.0 and 45°C, respectively. Na+, K+ and Li+ had no effect on the activity of the enzyme. Mg2+ activated the enzyme while Zn2+, Cu2+ inhibited its activity. The Km was 0.64mmol/L.
Keywords :
biochemistry; biological techniques; chromatography; enzymes; farming; liver; purification; ultrafiltration; Bos taurus; DEAE-sepharose column chromatography; alkaline phosphatase; cattle; enzyme; liver; n-butanol disposal; purification; sephacryl S-200 gel filtration chromatography; temperature 10.0 degC; temperature 45 degC; thermal denaturation; ultrafiltration; Agriculture; Biochemistry; Biological materials; Cows; Laboratories; Liver; Proteins; Purification; Refrigeration; Temperature measurement;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5515660
