Effect of Surface Modification for Protein Microarray on Immobilizing Probe

Glass slides were modified with (3-aminopropyl)-trimethoxysilane, glutaraldehyde, poly-L-lysine to fabricate protein microarrays. Evaluate and choose more optimal surface modification methods for the preparation of protein microarray. The proteins probe labeled by Cy3 was immobilized onto the activated glass slides, attachment rate was evaluated. Subsequently, the mouse IgG were printed on the modified glass slides, the target proteins were labeled by Cy3, responsive activity of protein on microarrays were evaluated. The attachment rate and the effects of immobilized proteins on the glass were compared. The result showed that the aldehyde modification is more suitable surface modification for the fabrication of protein microarrays.