Subcellular Localization of GFP-Tetherin Fusion Protein in COS-1 by Confocal Laser Scanning Microscope

Author

Yang Yishu ; Lin Xiaoyan ; Sun Xiaona ; Wang Xiaoli ; Wu Senren ; Xiao Xiangqian ; Li Zelin ; Zeng Yi

Author_Institution

Coll. of Life Sci. & Bioeng., Beijing Univ. of Technol., Beijing, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

3

Abstract

Tetherin cDNA was amplified from total RNA prepared from HeLa cells by RT-PCR, then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-Tetherin construct was transfected into COS-1 to determine the expression and the subcellular localization of GFP-Tetherin fusion protein. Under CLSM the localization of GFP-Tetherin fusion protein distributed outside the nucleus and co-localized with TGN in COS-1 cells.

Keywords

DNA; biomedical optical imaging; cellular biophysics; genetics; laser applications in medicine; molecular biophysics; optical microscopy; proteins; CLSM; COS-1 cell; GFP-tetherin fusion protein; HeLa cells; RNA; RT-PCR; confocal laser scanning microscope; eukaryotic expression vector pEGFP-C3; nucleus; subcellular localization; tetherin cDNA; Cells (biology); Cloning; Fluorescence; Laser fusion; Laser theory; Manufacturing; Microscopy; Proteins; RNA; Rabbits;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5515712

Filename

5515712