DocumentCode :
3107543
Title :
Preparation and Structure Analysis of Chondroitin Sulfate from Pig Laryngeal Cartilage
Author :
Li, Anlin ; Xiong, Shuangli
Author_Institution :
Dept. of Biosci. & Biotechnol., Southwest Univ. of Sci. & Technol., Mianyang, China
fYear :
2010
fDate :
18-20 June 2010
Firstpage :
1
Lastpage :
5
Abstract :
The chondroitin sulfate with high purity was extracted by papain hydrolysis, isolated and purified by tricloroacetic acid, alcohol precipitation, dialysis and DEAE-Sepharose Fast Flow ion exchange column chromatography. The structure was identified by biological enzymolysis and spectral analysis. GC data exhibited that neutral monosaccharide in chondroitin sulfate only included 0.84% of xylose and 1.28% of galactose. IR data showed that chondroitin sulfate included chondroitin-4-sulfate and chondroitin-6-sulfate isomer in a molecular chain. The results of enzyme hydrolysis and spectra analysis, including reverse phase high performance liquid chromatography, mass-spectrum and NMR, indicated that ChS from pig laryngeal cartilage contains the repeated units of GlcA(β1-3)GalNAc4SO3 and GlcA(β1-3)GalNAc6SO3. It may not have the structure characteristics of keratan sulfate and dermatan sulfate such as NeuAc (α2-3) Gal (β1-4) GlcNAc4SO3 units and GlcA2SO3 (β1-3) GalNAc4SO3, GlcA2SO3 (β1-3) GalNAc6SO3 units. This may explain the internal mechanism of its antioxidation.
Keywords :
biochemistry; biological tissues; biotechnology; enzymes; molecular biophysics; DEAE-Sepharose fast flow ion exchange column chromatography; IR spectra; NMR; alcohol precipitation; biological enzymolysis; chondroitin sulfate; dermatan sulfate; dialysis; high performance liquid chromatography; keratan sulfate; mass spectrum; neutral monosaccharide; papain hydrolysis; pig laryngeal cartilage; spectral analysis; tricloroacetic acid; Animal structures; Biological materials; Biotechnology; Data mining; Isolation technology; Magnetic analysis; Nuclear magnetic resonance; Protection; Purification; Spectral analysis;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
ISSN :
2151-7614
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
Type :
conf
DOI :
10.1109/ICBBE.2010.5515812
Filename :
5515812
Link To Document :
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