Study on Fermentation Technical Conditions of Pyrofomes demidoffii Mycelia

Pyrofomes demidoffii is a new recorded species of the new recorded genus in the China. To study the high yield and economical medium productive conditions, this experiment selected and used single factors such as carbon source, nitrogen source, C/N rations, rational speed, temperature, pH value, bottled volume and inoculated bulk and multifactor experiment design with submerged fermentation mycelia to explore the effects of these various factors on the mycelia yield. The results of the experiment indicated that mycelia of P. demidoffii could be produced by submerged fermentation process. Meanwhile starch and bran were the optimum carbon and nitrogen source; P, K, Mg had facilitation to the growth of mycelia. The optimal rotational speed for the growth of mycelia is 150 rpm, temperature 25~30, pH value 5~7, bottled volume 100 ml, and inoculated bulk 8%, C/N ratios 20:1~25:1. The optimal nutritional conditions when the mycelia of this sort of fungi were cultivated by means of submerged culture are follow: glucose 25 g·l^-1, bran 350 g·l^-1, K₂HPO₄ 0.25 g·l^-1, pH 6.5.The growth curve of the logistic equation was deduced: y = 0.22/(1+1.019e^-0.013x), the weight of the dry mycelia was 0.22 g·100 ml^-1.