Development of active matrix biosensor array for cell screening

Author

Huang, Xiaoqiu ; Nausieda, I. ; Greve, David W. ; Domach, Michael M. ; Nguyen, Duc

Author_Institution

Dept. of Electr. & Comput. Eng., Carnegie Mellon Univ., Pittsburgh, PA, USA

fYear

2004

fDate

24-27 Oct. 2004

Firstpage

72

Abstract

Measurements of the AC electrode impedance can be used to sense changes in cell adhesion to the electrode and also the cell response to drugs. In this paper, we report progress toward the further development of this sensing technology. We describe extended (∼72 hr) studies of the impedance changes of electrodes which monitor the cell growth under environmentally controlled conditions, and we contrast the signatures of the mouse fibroblast 3T3 and human colorectal carcinoma HCT-116 cell lines. We also describe the design and fabrication of a CMOS-based active matrix sensor array for statistical analysis of a large population of individual cells.

Keywords

CMOS integrated circuits; biological techniques; biological tissues; biomedical electrodes; biomedical electronics; biosensors; cancer; electric impedance; integrated circuit design; microelectrodes; microsensors; statistical analysis; 72 hr; AC electrode impedance measurements; CMOS-based active matrix sensor array; active matrix biosensor array; cell adhesion; cell drug response; cell growth monitor; cell screening; environmentally controlled conditions; human colorectal carcinoma HCT-116 cell line signature; impedance changes; individual cell population; mousefibroblast 3T3 cell line signature; sensing technology; statistical analysis; Active matrix technology; Adhesives; Biosensors; CMOS technology; Condition monitoring; Drugs; Electrodes; Impedance measurement; Pharmaceutical technology; Sensor arrays;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2004. Proceedings of IEEE

Print_ISBN

0-7803-8692-2

Type

conf

DOI

10.1109/ICSENS.2004.1426102

Filename

1426102