Diosmetin Substituent in Galium verum L. Acting on Venous Thrombosis

Galium verum L. is a wild plant from Northeast China, this grass is well known as an excellent food source of vitamin, and it also contains phytochemicals such as chlorogenic acid,salicylic acid and flavone. The present work evaluates the anti-venous thrombosis capacity of hydrophilic extracts of Galium. Anti-venous thrombosis activity values obtained for Galium were higher than those reported for other plant particularly rich in flavones. Diosmetin substituent compounds by high performance liquid chromatography (RPLC), as main factor responsible for anti-venous thrombosis activity, were determined. To investigation the proteome changes of the plasma, three groups of the wistar rats were selected, the controls group did not undergo any processing, the model group and the prevention group were established thrombosis model in vitro and the difference between them was the prevention group had been given diosmetin substituent before which the model group hadn´t. Plasma proteins were extracted by high velocity centrifugation and separated by Sephadex column to remove the high-abundance proteins such as immunoglobulin. The low-abundance of proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the images were analyzed by PDquest software.Comparative proteome was used in studying diosmetin substituent of venous thrombosis, several differential expression of proteins were found, and the functions of these proteins and expression trends were analyzed. Clarify the thrombolytic mechanism of diosmetin substituent acting on the vein in order and find new substances with thrombolytic specificity. There may be providing a theoretical basis to find a new ideal thrombolytic substance and promote the treatment of venous thrombosis.