DocumentCode :
3117716
Title :
Sensorized nanoliter reactor chamber for DNA multiplication
Author :
Iordanov, Ventzeslav P. ; Iliev, Blagoi P. ; Joseph, Victor ; Bossche, Andre ; Bastemeijer, Jeroen ; Sarro, Pasqualina M. ; Young, Ian T. ; Van Dedem, Gijs W K ; Vellekoop, Michiel J.
Author_Institution :
DIMES, Delft Univ. of Technol., Netherlands
fYear :
2004
fDate :
24-27 Oct. 2004
Firstpage :
229
Abstract :
This paper presents thermal analysis verification of a sensorized 50 nl reactor chamber for DNA amplification based on PCR (polymerase chain reaction). The reactor is equipped with an integrated heater, temperature sensor and a photo detector for real time detection. Through micromachining, the thermal capacity of each chamber is minimized, enabling rapid PCR cycling. The proposed structure was implemented on a silicon substrate using a standard CMOS process and postprocessing. The chambers have a bottom area of 500×500 μm2 and a pitch of 1 mm. An array of 96 reactors can be formed on a square centimeter. In order to reach the required PCR temperature levels (55°C, 75°C and 92°C) dedicated electronics, based on a proportional-integral (PI) controller were designed and built. The system is capable of stabilizing the temperature of the reactor and performing a temperature sweep up to 100°C.
Keywords :
CMOS integrated circuits; DNA; PI control; bioreactors; biosensors; microfluidics; micromachining; microsensors; photodetectors; temperature control; temperature sensors; 1 mm; 100 degC; 500 micron; 55 degC; 75 degC; 92 degC; CMOS process; DNA multiplication; PI controller; biochemical analysis; chamber bottom area; chamber thermal capacity; integrated heater; integrated photo detector; integrated temperature sensor; micromachining; polymerase chain reaction; proportional-integral controller; rapid PCR cycling; reactor temperature stabilization; sensorized nanoliter reactor chamber; temperature control; temperature sweep; CMOS process; DNA; Detectors; Inductors; Micromachining; Pi control; Polymers; Rapid thermal processing; Silicon; Temperature sensors;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2004. Proceedings of IEEE
Print_ISBN :
0-7803-8692-2
Type :
conf
DOI :
10.1109/ICSENS.2004.1426143
Filename :
1426143
Link To Document :
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