Title :
Utilization of 3D hyaluronan based scaffolds for in vitro cultures of rat sciatic nerve cells
Author :
Girotto, D. ; Zavan, B. ; Abatangelo, G.
Author_Institution :
Dept. of Histology, Microbiol. & Med. Biotechnol., Univ. of Padua, Italy
Abstract :
Primary sciatic rat nerve cells and nerve fragments can be in vitro cultured onto or into various forms of scaffolds made of benzyl ester of hyaluronan. Both isolated cells and cells outgrowing from nerve explants attach to the biomaterial, proliferate and migrate on its surface. Two cell populations are present: S-100 positive cells and fibroblast-like cells.
Keywords :
biological techniques; cellular biophysics; neurophysiology; organic compounds; 3D hyaluronan based scaffolds; S-100 positive cells; Schwann cells; benzyl ester; biomaterial; cells migration; cells proliferation; fibroblast-like cells; hyaluronan-based bimaterials; in vitro cultures; nerve explants; rat sciatic nerve cells; Biological materials; Biomembranes; Cells (biology); Fiber lasers; Fibroblasts; In vitro; Optical fiber devices; Optical materials; Surface morphology; Testing;
Conference_Titel :
Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on
Print_ISBN :
0-7803-7557-2
DOI :
10.1109/MCTE.2002.1175018
