Title :
Axonal regeneration through a bioresorbable HYAFF® based conduit in the peripheral nervous system of the rat
Author :
Bunting ; Hall, S.
Author_Institution :
Centre for Neurosci. Res., GKT Sch. of Biomed. Sci., London, UK
Abstract :
Summary form only given. Axonal regeneration across a 0.5 cm interstump gap in the sciatic nerve of adult Wistar rats was compared using 3 experimental models, namely a HYAFF® based bioresorbable conduit, a peripheral nerve autograft and an unfilled gap. Tissue was harvested at 1, 2 and 4 weeks post operatively, fixed in 4% paraformaldehyde and processed through polyester wax. 7μm longitudinal sections of the implant site and stumps were immunostained using a panel of antibodies to identify Schwann cells and regenerating axons. Reinnervation of the distal stump was quantified 1 cm distal form the implant site.
Keywords :
biomedical materials; cellular biophysics; neurophysiology; 0.5 cm; 1 cm; 1 to 4 w; 7 micron; Schwann cells identification; axonal regeneration; bioresorbable HYAFF® based conduit; distal stump reinnervation; immunostained stumps; implant site; paraformaldehyde; polyester wax; regenerating axons; sciatic nerve; unfilled gap; Bridges; Immune system; Implants; Nerve fibers; Nervous system; Neuroscience; Rats;
Conference_Titel :
Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on
Print_ISBN :
0-7803-7557-2
DOI :
10.1109/MCTE.2002.1175058
