Title :
Purification and Characterization of Cathepsins from the Gut of Apostichopus japonicus
Author :
Han, Bing ; Han, Xue ; Zhu, Beiwei
Author_Institution :
Collage of Food Sci. & Eng., Harbin Univ. of Commerce, Harbin, China
Abstract :
Cathepsins from gut of apostichopus japonicus were prepared by ammonium sulfate fractionation, purified by DEAE-52 and Sephadex-100 chromatographic separations. Cathepsins were purified more than 66 folds and yield reduced to 1.7%. Optimal pH and temperature were 7.0 and 35°C, respectively.
Keywords :
biochemistry; proteins; purification; DEAE-52; Sephadex-100; ammonium sulfate; apostichopus japonicus; cathepsins; chromatographic separations; fractionation; temperature 35 degC; Biochemistry; Business; Chemicals; Degradation; Educational institutions; Fractionation; Pollution measurement; Proteins; Purification; Temperature distribution;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5516401
