Chondrogenesis of Mesenchymal Stem Cells by Controlled Delivery of Transforming Growth Factor-Ã\x9f3

Author

Moioli, Eduardo K. ; Mao, Jeremy J.

fYear

2006

fDate

Aug. 30 2006-Sept. 3 2006

Firstpage

2647

Lastpage

2650

Abstract

Human mesenchymal stem cells (hMSC) have been shown to differentiate into chondrocytes and form cartilage-like tissues when cultured with TGFbeta3 at 10 ng/ml. Previous attempts to engineer cartilage using hMSC have depended on in vitro pre-differentiation in order to form chondrogenic engineered constructs. Such techniques greatly increase the time of implant fabrication and suffer from loss of phenotype upon withdrawal from chondrogenic medium. The present study investigates a tissue engineered construct that includes sustained delivery of TGFbeta3 and induces differentiation of hMSC into chondrocytes in situ using injectable thermosensitive gels

Keywords

biomedical materials; bone; cellular biophysics; orthopaedics; tissue engineering; cartilage-like tissues formation; cell culture; cell differentiation; chondrocytes; chondrogenesis; chondrogenic engineered constructs; controlled delivery; engineered cartilage; growth factor-beta3; human mesenchymal stem cells; implant fabrication; in vitro pre-differentiation; injectable thermosensitive gels; tissue engineered construct; Arthritis; Bones; Cities and towns; Extracellular; Implants; In vitro; Joints; Stem cells; Temperature; USA Councils;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE

Conference_Location

New York, NY

ISSN

1557-170X

Print_ISBN

1-4244-0032-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2006.260861

Filename

4462340