Title :
Chondrogenesis of Mesenchymal Stem Cells by Controlled Delivery of Transforming Growth Factor-Ã\x9f3
Author :
Moioli, Eduardo K. ; Mao, Jeremy J.
fDate :
Aug. 30 2006-Sept. 3 2006
Abstract :
Human mesenchymal stem cells (hMSC) have been shown to differentiate into chondrocytes and form cartilage-like tissues when cultured with TGFbeta3 at 10 ng/ml. Previous attempts to engineer cartilage using hMSC have depended on in vitro pre-differentiation in order to form chondrogenic engineered constructs. Such techniques greatly increase the time of implant fabrication and suffer from loss of phenotype upon withdrawal from chondrogenic medium. The present study investigates a tissue engineered construct that includes sustained delivery of TGFbeta3 and induces differentiation of hMSC into chondrocytes in situ using injectable thermosensitive gels
Keywords :
biomedical materials; bone; cellular biophysics; orthopaedics; tissue engineering; cartilage-like tissues formation; cell culture; cell differentiation; chondrocytes; chondrogenesis; chondrogenic engineered constructs; controlled delivery; engineered cartilage; growth factor-beta3; human mesenchymal stem cells; implant fabrication; in vitro pre-differentiation; injectable thermosensitive gels; tissue engineered construct; Arthritis; Bones; Cities and towns; Extracellular; Implants; In vitro; Joints; Stem cells; Temperature; USA Councils;
Conference_Titel :
Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE
Conference_Location :
New York, NY
Print_ISBN :
1-4244-0032-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2006.260861
