DocumentCode :
3121232
Title :
Identification of a High Phosphate-Accumulating Organisms and Study of Its Poly-Phosphate Characteristics
Author :
Yang, Junzhong ; Yuan, Liling ; Xu, Shangying ; Zhu, Derui ; Ni, Yan ; Yuan, Yongze ; Liu, Deli ; Yang, Jiangke
Author_Institution :
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Central China Normal Univ., Wuhan, China
fYear :
2010
fDate :
18-20 June 2010
Firstpage :
1
Lastpage :
4
Abstract :
15 bacterial strains with a capacity of poly-phosphate were isolated from enrichment culture of the soil sample collected from the phosphate fertilizer factory of Honghu City in Hubei province, of which one strain named as HS-P7 has the strongest effects of poly-phosphate. Ammonium molybdate spectrophotometric method was used to measure its ability of poly-phosphate. The results showed that phosphorus removal rate about the strain HS-P7 reached to 90.08% when it was cultivated in the phosphorus-rich medium in 30°C during 72 hours. The homology between the strain HS-P7 and Pseudomonas cf. Monteilii is 98% by comparing their 16S rDNA. Based on the characteristics of morphological, physiological and biochemical, the strain HS-P7 was identified as Pseudomonas sp. The effects of different temperatures, pH, carbon sources and nitrogen sources on the growth of the strain HS-P7 were investigated and the optimum growth condition was determined as pH 7.0 and 30°C.
Keywords :
DNA; biochemistry; biological techniques; cellular biophysics; microorganisms; molecular biophysics; molecular configurations; pH; phosphorus compounds; soil; 16S rDNA; Pseudomonas cf. Monteilii; ammonium molybdate spectrophotometric method; bacterial strains; biochemical characteristics; carbon sources; high phosphate-accumulating organisms; morphological characteristics; nitrogen sources; optimum growth condition; pH; phosphate fertilizer factory; phosphorus removal rate; phosphorus-rich medium; physiological characteristics; polyphosphate characteristics; soil sample; strain HS-P7; temperature 30 degC; temperature effect; time 72 h; Capacitive sensors; Isolation technology; Microorganisms; Nitrogen; Organisms; Soil; Temperature; Wastewater; Water pollution; Water storage;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
ISSN :
2151-7614
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
Type :
conf
DOI :
10.1109/ICBBE.2010.5516450
Filename :
5516450
Link To Document :
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