Title :
Single molecule recognition of SGLT1 in live G6D3 cells using force spectroscopy
Author :
Puntheeranurak, Theeraporn ; Michel, Kirsten ; Scharlau, Daniel ; Kinne, Rolf K H ; Hinterdorfer, P.
Author_Institution :
Inst. for Biophys., Johannes Kepler Univ., Linz, Austria
Abstract :
SGLT1, a member of the sodium-dependent glucose co-transporter family, plays a crucial role in the re-absorption of filtered D-glucose in the kidney proximal tubule. The present study extends the AFM investigations to a live cell system in order to obtain direct evidence of SGLT1 recognition in intact cell membranes.
Keywords :
atomic force microscopy; biomembrane transport; biosensors; kidney; sugar; AFM; SGLT1; filtered D-glucose re-absorption; force spectroscopy; intact cell membranes; kidney proximal tubule; live G6D3 cells; single molecule recognition; sodium-dependent glucose co-transporter; Atomic force microscopy; Biomembranes; Biophysics; Brushes; Cells (biology); Kinetic theory; Physiology; Proteins; Spectroscopy; Sugar;
Conference_Titel :
Sensors, 2004. Proceedings of IEEE
Print_ISBN :
0-7803-8692-2
DOI :
10.1109/ICSENS.2004.1426404
